Guidelines for Submission of Samples
for Molecular Diagnostic Testing
- Peripheral venous blood
- Amniotic fluid cells
- Chorionic villi
- Other clinical samples
Peripheral venous blood
Blood samples should be collected in EDTA tubes (purple/lavender top) and well mixed. In general, 2 x 5-7 ml draw tubes (eg. B-D Vacutainer brand #6452) from individuals 10 years of age or older. Younger individuals need only provide 5 ml blood samples as above, while newborn infants require only 1 - 2 ml samples. These blood samples provide a large amount of DNA and remaining DNA will be stored after testing in the CompGene DNA bank. Blood samples may be collected in acid-citrate-dextrose (ACD) tubes (yellow top) but these are not preferred.
Please do not use heparin tubes (green top) as this anti-coagulant may cause problems with subsequent DNA analysis.
After blood sample collection, the tubes can be safely stored in the refrigerator until shipped. The samples should be shipped by overnight courier in appropriate packaging under ambient conditions as soon as possible after collection. Please protect from freezing.
Amniotic fluid cells
Amniotic fluid should be collected into sterile centrifuge tubes (for example, Falcon 15/50 ml conical). Only 10-15 ml (or less for some tests) are required for most tests but if you would like also to have cytogenetic studies performed, please ship 15 - 20 ml of amniotic fluid. The samples should be protected by styrofoam packaging and shipped by courier as soon as possible at ambient temperature. Please protect from freezing.
NB If a bloody tap is obtained, please provide a sample of maternal peripheral blood to permit appropriate testing to confirm fetal cell sampling.
CompGene can provide (at an additional cost) tissue culture services for amniotic fluid cells. Alternatively, please provide two confluent T25 flasks of amniocytes. The samples should be protected by Styrofoam packaging and shipped by courier as soon as possible at ambient temperature. Please protect from freezing.
Chorionic villi should be collected into sterile tissue culture medium in a sterile culture tube and shipped by courier in appropriate packaging for overnight delivery at ambient temperature. Please protect from freezing. A sample of maternal peripheral blood must be provided to permit appropriate testing to confirm fetal cell sampling.
Other clinical samples
CompGene has had experience with a wide range of unusual clinical samples including archival pathology specimens such as tissue sections on slides or blocks of paraffin-embedded tissues. Molecular genetic diagnoses are sometimes possible on old specimens (> 15 years old). Please consult with CompGene prior to sending any samples other than those described above.
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